Agarose gel showing Pasteurella multocida PCR amplified product of 460 bp for kmT1 gene using specific primer.
Lane L: marker, 100bp DNA ladder.
Lane 1, 3, 4, 6, 8, 10, 11, 12, 14, and 20: strains belong to Pasteurella multocida isolates. 

Multiplex polymerase chain reaction for capsular typing.
Lane L: marker, 100bp DNA ladder.
Lane1, 3, 4, 6, 8, 10, 11, 12, 14, and 20: strains belong to capsular A type. 

Agarose gel electrophoresis of multiplex PCR of pfhA (275 bp), hgbB (499 bp), tbpA (728 bp), and toxA (846 bp) virulence genes for characterization of Pasteurella multocida.
Lane M: 100 bp ladder as a molecular size DNA marker. Lane C+: Control positive for pfhA, hgbB, tbpA and toxA genes. Lane C-: Control negative. Lanes 1, 3, 4, 5, 7 & 10: Positive strains for hgbB gene. Lanes 2, 8 & 9: Positive strains for pfhA gene. Lane 2: Positive strain for tbpA gene.
Lane 10: Positive strain for toxA gene. Lane 6: Negative strain for pfhA, hgbB, tbpA and toxA genes. 

Agarose gel electrophoresis of multiplex PCR of sodA (361 bp) and ptfA (488 bp) virulence genes for characterization of Pasteurella multocida.
Lane M: 100 bp ladder as a molecular size DNA marker. Lane C+: Control positive for ptfA and sodA genes. Lane C-: Control negative. Lanes 1, 2, 5, 6, 7 & 9: Positive strains for ptfA and sodA genes. Lanes 4, 8 & 10: Positive strain for ptfA gene. Lanes 3: Positive strain for sodA gene 

Agarose gel electrophoresis of multiplex PCR of sodC (235 bp), nanB (544 bp), and oma87 (948 bp) virulence genes for characterization of Pasteurella multocida.
Lane M: 100 bp ladder as a molecular size DNA marker. Lane C+: Control positive for sodC, nanB, and oma87 genes. Lane C-: Control negative. Lanes 1, 2, 3, 4, 6, 8, 9 & 10: Positive strains for sodC, nanB, and oma87 genes. Lane 5: Positive strain for nanB and oma87 genes. Lane 7: Positive strain for sodC and oma87 genes.