Uniplex PCR for 16s rRNA detection of E. coli, Lane M:100-3000bpDNA marker; Lanes 1-10 were positive E. coli isolates. 

PCR for detection of rfbEO157 encoding gene. Lane M:100-3000bpDNA marker; Lane 1, 2: positive isolate for rfbEO157 gene; Lanes 3-7: negative strains. 

Multiplex PCR for detection of blaTEM, blaCTXM, blaSHV and blaOXA genes in E. coli isolates. Lane M:100-3000bpDNA marker; Lane 1 positive blaTEM& blaSHV at 445, 237bp, respectively; Lanes 3,9 positive blaTEM at 445bp; Lane 4 positive blaCTXM, blaSHV at 593, 237bp, respectively; Lanes 5-8&10 positive strains for blaTEM & blaCTXM at 445 and 593bp; Lane 2 negative sample. 

Multiplex PCR for mcr1 and blacmyІІ genes detection, Lane M:100-3000bpDNA marker; Lanes 2,5, 6 and 7 were positive blacmyІІ at 695bp, while Lane 14 positive mcr1 and blacmyІІ at 320, 695bp, respectively. Lanes 1,3, 4 and 8-13 are negative samples. 

Antibiotic sensitivity testing of E. coli isolates.