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Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 3 (1S): 16 -21. Special Issue-1 (Biotechnological and molecular approaches for diagnosis, prevention and control of infectious diseases of animals)
 
Figure 1A

Detection of amplification of cDNA of VP7 genes of buffalo rotavirus in l% agarose gel electrophoresis of buffalo rotavirus Isolates by RT- PCR
Lanes: M- 100 bp DNA Ladder, 1-11: BoRVA VP7 gene products, 12: no template control.

 

Figure 1B

Detection of amplification of cDNA of VP4 genes of buffalo rotavirus in l % agarose gel electrophoresis of buffalo rotavirus Isolates by RT- PCR
Lanes: M- 100 bp DNA Ladder, 1-11: BoRVA VP4 gene products, 12-13: no template control.

 

Figure 2

P genotyping of the buffalo rotavirus using VP4 gene type specific primers by semi-nested multiplex PCR
Lanes: M- 100 bp DNA Ladder, 1-4: BoRVA P[11] genotype specific PCR products. 

Figure 3

G genotyping of the buffalo rotavirus using VP7 genotypes specific primers by semi-nested multiplex PCR
Lanes: M- 100 bp DNA Ladder; Lanes: 1 and 8 BoRVA G6 genotype specific products; 2: no template control; 3-7: BoRVA G10 genotype specific products.